Super-resolved fluorescence microscopy, Dr. Paolo Ferriani (Uni Kiel)

23.06.2015 von 17:00 bis 18:00

Gastgeber: Prof. Heinze

For a long time optical microscopy was held back by a presumed limitation: that it would never obtain a better resolution than half the wavelength of light. This, known as Abbeʼs diffraction limit, had seemed to be an insurmountable barrier to using microscopes to see features in biological cells that are smaller than a few hundred nanometers. S. Hell, E. Betzig, and W. Moerner - Nobel Laureates in 2014 - have demonstrated that the diffraction limit can be circumvented with the help of fluorescent molecules. Their work has brought optical microscopy into the nanodimension.

In this lecture I will describe the working principles of the two methods of super-resolved fluorescence microscopy. First, the stimulated emission depletion microscopy, developed by S. Hell, which utilizes two laser beams - one stimulates fluorescent molecules to glow, another cancels out all fluorescence except for that in a nanometre-sized volume. Second, the single-molecule microscopy, invented by E. Betzig, and W. Moerner, which relies on the possibility to turn the fluorescence of individual molecules on and off. I will then provide examples of applications of such techniques.

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